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KMID : 1094720100150030383
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Biotechnology and Bioprocess Engineering
2010 Volume.15 No. 3 p.383 ~ p.391
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Evaluation and Optimization of Factors Affecting the Performance of a Flow-through System Based on Immobilized Acetylcholineesterase as a Biosensor
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Ebrahimi Bahman
Shojaosadati Seyed Abbas
Mohammad Seyyed
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Abstract
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A flow-through system based on acetylcholineesterase (AChE) was studied. The system was prepared by mixing AChE and a multiwall carbon nanotube (MWCNT). Two important parameters, the ratios of AChE:MWCNT (X1) and AChE-MWCNT:sol-gel (X2) were optimized using response surface methodology. The results revealed that an enzyme immobilized within the MWCNT-sol-gel was more effective compared to one conducted with sol-gel. The optimum feed flow rate was 0.4 mL/min and ATChI concentration was found to be 1 mM. The optimum ratios of X1 and X2 for immobilization on ceramic packing were 1.07 and 0.43, respectively. The sensitivity of this flow-through system was 1.82 ? 10?5/꺤M and long-term stability analyzed after 120 days was 74% of initial absorbance. With respect to an incubation time of 14 min, the detection limit for paraoxon was 7.3 ? 10?12 mol.
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KEYWORD
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flow-through system, optimization, acetylcholine, multiwall carbon nanotube, sol-gel, ceramic packing
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